[软物质物理重点实验室系列学术报告（106）] A backtracked and paused transcription
||Dr. Shuang Wang (王爽)
Institut Jacques Monod, University Paris Diderot, France.
Promoter escape is a highly regulated, rate-limiting step in transcription initiation. Using magnetic tweezers, we studied the kinetics of promoter escape in E. Coli system. Results showed that an intermediate of the RNA polymerase (RNAP) does exist prior to promoter escape, and the RNAP stalled in this intermediate can be efficiently rescued in the presence of GreA factor. In real time, RNAP adopted a long-lived backtracked state during transcription initiation and that the paused-backtracked intermediate was populated abundantly at physiologically relevant nucleoside triphosphate (NTP) concentrations. Our results confirm the existence of a previously hypothesized paused and backtracked RNAP initiation intermediate and suggest it is biologically relevant; furthermore, such intermediates could be exploited for therapeutic purposes and may reflect a conserved state among paused, initiating eukaryotic RNA polymerase II enzymes.
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